British number one Kyle Edmund reacted angrily to noise from the crowd during his Miami Open fourth-round defeat by American John Isner.
He was trailing 5-3 in the second set tie-break when the point was stopped after he heard a shout from the crowd.
Umpire Carlos Bernardes ruled Edmund had lost the point as a result, with the Briton saying: “They keep shouting every point, I keep hearing it.”
Isner served an ace on the next point to wrap up a 7-6 (7-5) 7-6 (7-3) win.
Eighth seed Isner will face either world number one Novak Djokovic or Spain’s Roberto Bautista Agut next.
Australia’s Nick Kyrgios was given a point penalty for appearing to swear at a member of the crowd during his loss to Borna Coric.
Kyrgios, who was abused by a spectator in his third-round win over Dusan Lajovic, also smashed his racquet in the 4-6 6-3 6-2 defeat.
He was given a warning for breaking the racquet – which he later gave to a crowd member – before being deducted a point for an audible obscenity.
The 23-year-old criticised umpire Gianluca Moscarella at a changeover, saying: “The entire match people were screaming out. You did not do one thing until I told you to do it.
“Honest feedback, you’ve done a terrible job.”