Britain’s Harriet Dart lost to Australian world number 47 Ajla Tomljanovic in the last 16 of the Nature Valley Open in Nottingham.
Dart, ranked 159th in the world, lost 6-7 (5-7) 6-1 6-2 in her second grass-court match of the season.
The 22-year-old was broken twice in the opening set but won the tie-break before losing the second and third.
She failed to convert four break points, including two at 2-2 in the deciding set.
Maia Lumsden, the only other remaining Briton in the women’s singles, was beaten 6-3 6-1 by French world number 28 Caroline Garcia.
In the second-tier Challenger event taking place at the same venue, British number three Dan Evans beat compatriot Jack Draper 7-5 6-3 to make the last 16.
Evans, who beat Serbia’s Viktor Troicki in the Surbiton final on Sunday, will play Sweden’s Elias Ymer next. The 29-year-old, is currently ranked 70th in the world as he continues to climb the rankings following a year’s ban from the sport for taking cocaine.
- Evans focused on the future
On Wednesday British number three Heather Watson lost 6-4 6-3 to Greece’s Maria Sakkari, ranked 82 places higher, in the first round in rainy conditions.
Compatriot Katie Swan also lost to American Bernarda Pera 7-6 (7-5) 6-1.